Article

Selection of internal references for transcriptomics and RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell lines

2020-10-22

Abstract excerpt

Transcriptomics has been widely applied in uncovering disease mechanisms and screening potential biomarkers. Internal reference selection determines the accuracy and reproducibility of data analyses. The aim of this study was to identify the most qualified reference genes for the relative quantitation analysis of transcriptomics and real-time quantitative reverse-transcription PCR in fourteen NF1 related cell line...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 320ff483-2139-5cd0-b243-5d56fda0bf6c
DOI: 10.1101/2020.10.22.350017

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Selection of internal references for transcriptomics and RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell linesDOI 10.1101/2020.10.22.350017Read

Selection of suitable reference genes for quantitative real-time polymerase chain reaction in human meningiomas and arachnoideaSemantic proximity Read

RNA-Seq is not required to determine stable reference genes for qPCR normalizationSemantic proximity Read

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read

Normalization Strategy for Selection of Reference Genes for RT-qPCR Analysis in Left Ventricles of Failing Human HeartsSemantic proximity Read

Evaluation of reference genes suitable for studying mRNAs and microRNAs expression in thyroid neoplasmsSemantic proximity Read

Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCRSemantic proximity Read

Identification and Validation of Quantitative PCR Reference Genes Suitable for Normalizing Expression in Normal and Dystrophic Cell Culture Models of MyogenesisSemantic proximity Read

Selection of appropriate reference genes for normalization of qRT-PCR based gene expression analysis in SARS-CoV-2, and Covid-associated Mucormycosis infectionSemantic proximity Read

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell linesSemantic proximity Read

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.Semantic proximity Read

Validation of Reference Genes for Oral Cancer Detection Panels in a Prospective Blinded Cohort.Semantic proximity Read

Screening and validation of suitable internal reference genes for gene expression analysis in Rubia cordifolia L. under different abiotic stressesSemantic proximity Read