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Article

Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

20 Oct 2011

Abstract excerpt

BACKGROUND: The choice of reliable reference genes is a prerequisite for valid results when analyzing gene expression with real-time quantitative PCR (qPCR). This method is frequently applied to study gene expression patterns in immune cells, yet a thorough validation of potential reference genes is still lacking for most leukocyte subtypes and most models of their in vitro stimulation. In the current study, we...

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PMID: 22011438
DOI: 10.1186/1756-0500-4-427

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