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Article

Reference genes in real-time PCR.

Journal of applied genetics - 1 Nov 2013

Kozera Bartłomiej, Rapacz Marcin

Abstract excerpt

This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name. Lots of papers report that in different conditions, for different organisms and even tissues the basic assumption—the constant level of the expression is not...

Topics

Algorithms
Gene Expression Regulation
Gene Expression Regulation, Plant
Genes, Plant
Genetic Variation
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
Plants
RNA, Ribosomal, 18S
Real-Time Polymerase Chain Reaction
Reference Standards

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reproducibility of Results

Selection, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 24078518
DOI: 10.1007/s13353-013-0173-x
PMCID: PMC3825189

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