Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.

PloS one - 1 Jan 2014

Thomas Kristen C, Zheng Xi Fiona, Garces Suarez Francia, Raftery Joanna M, Quinlan Kate G R, Yang Nan, North Kathryn N, Houweling Peter J

Abstract excerpt

The ability to obtain accurate and reproducible data using quantitative real-time Polymerase Chain Reaction (RT-qPCR) is limited by the process of data normalization. The use of 'housekeeping' or 'reference' genes is the most common technique used to normalize RT-qPCR data. However, commonly used reference genes are often poorly validated and may change as a result of genetic background, environment and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genetic Techniques
Share to Mice, Knockout

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Muscle, Skeletal

Share to Oligonucleotide Array Sequence Analysis

Share to Real-Time Polymerase Chain Reaction

Share to Reference Standards

Share to Reproducibility of Results

Share to Species Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 24523926
DOI: 10.1371/journal.pone.0088653
PMCID: PMC3921188

Genes

ACTN3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.PMID 24523926Read ↗

RNA-Seq is not required to determine stable reference genes for qPCR normalizationSemantic proximity Read ↗

Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restrictionSemantic proximity Read ↗

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular DystrophySemantic proximity Read ↗

Normalization Strategy for Selection of Reference Genes for RT-qPCR Analysis in Left Ventricles of Failing Human HeartsSemantic proximity Read ↗

Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilageSemantic proximity Read ↗

Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat modelSemantic proximity Read ↗

Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in craniosynostosis and bone-related tissues and cellsSemantic proximity Read ↗

Validation of Reference Genes for the Relative Quantification of Gene Expression in Human Epicardial Adipose TissueSemantic proximity Read ↗

Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studiesSemantic proximity Read ↗

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read ↗

Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research.Semantic proximity Read ↗

Identification and Validation of Quantitative PCR Reference Genes Suitable for Normalizing Expression in Normal and Dystrophic Cell Culture Models of MyogenesisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.