Article

Adaptation of a commercial NAD quantification kit to assay the base exchange activity of SARM1

2023-12-28

Abstract excerpt

<h4>ABSTRACT</h4> Here we report an adapted protocol using the Promega NAD/NADH-Glo™ Assay kit. The assay normally allows quantification of trace amounts of both oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) by enzymatic cycling, but we now show that the NAD analog 3- acetylpyridine adenine dinucleotide (AcPyrAD) also acts as a substrate. In fact, AcPyrAD generates amplification signals of...

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Identifiers and source

Literature Corpus work: c128182f-95bd-52ea-9908-f854f306289e
DOI: 10.1101/2023.12.28.573537

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