Article

Cytoplasmic tail truncation of SARS-CoV-2 Spike protein enhances titer of pseudotyped vectors but masks the effect of the D614G mutation

2021-06-22

Abstract excerpt

<h4>ABSTRACT</h4> The high pathogenicity of SARS-CoV-2 requires it to be handled under biosafety level 3 conditions. Consequently, Spike protein pseudotyped vectors are a useful tool to study viral entry and its inhibition, with retroviral, lentiviral (LV) and vesicular stomatitis virus (VSV) vectors the most commonly used systems. Methods to increase the titer of such vectors commonly include concentration by ul...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bfacca00-911b-540c-8c31-6b35edc58706
DOI: 10.1101/2021.06.21.449352

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Cytoplasmic tail truncation of SARS-CoV-2 Spike protein enhances titer of pseudotyped vectors but masks the effect of the D614G mutationDOI 10.1101/2021.06.21.449352Read

Cytoplasmic Tail Truncation of SARS-CoV-2 Spike Protein Enhances Titer of Pseudotyped Vectors but Masks the Effect of the D614G Mutation.Semantic proximity Read

SARS-CoV-2 Spike Alterations Enhance Pseudoparticle Titers and Replication-Competent VSV-SARS-CoV-2 VirusSemantic proximity Read

A method for the generation of pseudotyped virus particles bearing SARS coronavirus spike protein in high yieldsSemantic proximity Read

SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike ParticlesSemantic proximity Read

SARS CoV-2 nucleoprotein enhances the infectivity of lentiviral spike particlesSemantic proximity Read

Optimized Pseudotyping Conditions for the SARS-COV-2 Spike GlycoproteinSemantic proximity Read

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 SettingSemantic proximity Read

Optimized pseudotyping conditions for the SARS-COV2 Spike glycoproteinSemantic proximity Read

SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity.Semantic proximity Read

Lentiviral vector-based SARS-CoV-2 pseudovirus enables analysis of neutralizing activity in COVID-19 convalescent plasmaSemantic proximity Read

Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cellsSemantic proximity Read

Developing Pseudovirus-Based Neutralization Assay against Omicron-Included SARS-CoV-2 VariantsSemantic proximity Read