Article
Cytoplasmic Tail Truncation of SARS-CoV-2 Spike Protein Enhances Titer of Pseudotyped Vectors but Masks the Effect of the D614G Mutation.
Journal of virology - 27 Oct 2021
Chen Hsu-Yu, Huang Chun, Tian Lu, Huang Xiaoli, Zhang Chennan, Llewellyn George N, Rogers Geoffrey L, Andresen Kevin, O'Gorman Maurice R G, Chen Ya-Wen, Cannon Paula M
Abstract excerpt
The high pathogenicity of SARS-CoV-2 requires it to be handled under biosafety level 3 conditions. Consequently, Spike protein-pseudotyped vectors are a useful tool to study viral entry and its inhibition, with retroviral, lentiviral (LV), and vesicular stomatitis virus (VSV) vectors the most commonly used systems. Methods to increase the titer of such vectors commonly include concentration by ultracentrifugation...
Topics
- Animals
- Antibodies, Neutralizing
- Cell Line
- Genetic Vectors
- Humans
- Lentivirus
- Mutation
- SARS-CoV-2
- Spike Glycoprotein, Coronavirus
- Vesicular stomatitis Indiana virus
- Viral Load