Article

Optimized CRISPR/Cas9 Electroporation and Single Cell Cloning Protocol for Generating Pure Cellular Models in Human Immortalized Myoblasts

2025-09-08

Abstract excerpt

<h4>Background</h4> Genome editing in human skeletal muscle research requires protocols that maximize delivery while preserving viability and clonal outgrowth. We sought to develop a reagent-free workflow for CRISPR/Cas9 editing in human immortalized myoblasts and to demonstrate its performance in two use cases, an IARS1 knockout and an MLIP homozygous knock-in. <h4>Methods</h4> We optimized electroporation p...

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Literature Corpus work: b93155a0-4b43-57b1-976a-698355741b18
DOI: 10.1101/2025.09.08.674725

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