Article

Simplified In Vitro Generation of Human Gastruloids for Modelling Early Development

2025-10-15

Abstract excerpt

The purpose of this study was to optimize the efficiency and cost of human gastruloid formation by testing and adjusting individual parameters using as examples two distinct human pluripotent stem cell lines, both available from the UK Stem Cell Bank. For the first step, commercially sourced culture medium was replaced with a home-made defined recipe, known as ‘N2B27’, into which specific reagents can be titrated....

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Literature Corpus work: b3f9e3a7-c9e2-55c4-a8de-0553748ac79b
DOI: 10.1101/2025.10.15.682610

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