Article

Comparison of direct cDNA and PCR-cDNA Nanopore sequencing of <i>Escherichia coli</i> isolates

2024-01-23

Abstract excerpt

2. Whole-transcriptome (long-read) RNA sequencing (Oxford Nanopore Technologies, ONT) holds promise for agnostic analysis of differential gene expression (DGE) in pathogenic bacteria, including for antimicrobial resistance genes (ARGs). However, direct cDNA ONT sequencing requires large concentrations of polyadenylated mRNA, and amplification protocols may introduce technical bias. Here we evaluated the impact of...

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Literature Corpus work: afef1fa0-0520-5c03-8c58-ba0b03ba503f
DOI: 10.1101/2024.01.23.576853

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Comparison of direct cDNA and PCR-cDNA Nanopore sequencing of <i>Escherichia coli</i> isolatesDOI 10.1101/2024.01.23.576853Read

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