Article

Interrogating the <i>Escherichia coli</i> epitranscriptome via CRISPR interference and Nanopore native RNA sequencing

2026-04-13

Abstract excerpt

<h4>ABSTRACT</h4> Epitranscriptomics has recently gained significant momentum due to technological advances and translational applications, however, studies on bacterial RNA modifications remain limited. Bacterial RNA remains notoriously prone to degradation and methodologies to investigate the epitranscriptome are challenging. Prior research has shown RNA modifications modulate antimicrobial resistance, virulenc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e4ccff1d-6eec-568b-b128-998dc464c97e
DOI: 10.64898/2026.04.13.718120

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Interrogating the <i>Escherichia coli</i> epitranscriptome via CRISPR interference and Nanopore native RNA sequencingDOI 10.64898/2026.04.13.718120Read

The Small RNA MicC is a Multifunctional Regulator of Extraintestinal Pathogenic <i>Escherichia coli</i> Fitness across Multiple Host NichesSemantic proximity Read

Multiplex CRISPRi-Cas9 silencing of planktonic and stage-specific biofilm genes in <i>Enterococcus faecalis</i>Semantic proximity Read

tRNA modification profiling reveals epitranscriptome regulatory networks in <i>Pseudomonas aeruginosa</i>Semantic proximity Read

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read

Genome-scale CRISPRi profiling reveals metabolic vulnerabilities of uropathogenic <i>Escherichia coli</i> in human urineSemantic proximity Read

A broad-host-range CRISPRi toolkit for silencing gene expression in <i>Burkholderia</i>Semantic proximity Read

The tRNA epitranscriptomic landscape and RNA modification enzymes in <i>Vibrio cholerae</i>Semantic proximity Read

Transcriptome-based design of antisense inhibitors re-sensitizes CRE <i>E. coli</i> to carbapenemsSemantic proximity Read

Targeted sequencing of <i>Enterobacterales</i> bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore TechnologiesSemantic proximity Read

tRNA modification profiling reveals epitranscriptome regulatory networks in Pseudomonas aeruginosaSemantic proximity Read

RlmQ: A Newly Discovered rRNA Modification Enzyme Bridging RNA Modification and Virulence Traits in <i>Staphylococcus aureus</i>Semantic proximity Read

Adaptor Molecules Epitranscriptome Reprograms Bacterial PathogenicitySemantic proximity Read