Article

Pre-Sequencing Assessment of RNA-Seq Library Quality Using Real-Time qPCR

2025-07-11

Abstract excerpt

RNA sequencing (RNA-Seq) is an essential sequencing assay for studying transcriptome profiling. Ribosomal RNA (rRNA) comprises more than 80 - 90% of total cellular RNA, efficient rRNA removal is essential for accurately capturing the transcriptome, particularly to sequence low-abundance mRNAs. Inefficient rRNA removal during library preparation can result from variations in sample quality, preparation methods and...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f12ff238-549c-5dd4-8f7a-a86606dc2d1c
DOI: 10.1101/2025.07.08.663710

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Pre-Sequencing Assessment of RNA-Seq Library Quality Using Real-Time qPCRDOI 10.1101/2025.07.08.663710Read

Cross-Site Comparison of Ribosomal Depletion Kits for Illumina RNAseq Library ConstructionSemantic proximity Read

Comprehensive analysis of RNA-seq kits for standard, low and ultra-low quantity samplesSemantic proximity Read

Blocking abundant RNA transcripts by high-affinity oligonucleotides during transcriptome library preparationSemantic proximity Read

Systematic assessment of long-read RNA-seq methods for transcript identification and quantificationSemantic proximity Read

Comparative analysis of RNA enrichment methods for preparation of <i>Cryptococcus neoformans</i> RNA sequencing librariesSemantic proximity Read

Quantification and quality control of a small non-coding RNA preparation.Semantic proximity Read

IVT-seq reveals extreme bias in RNA-sequencingSemantic proximity Read

Poly(A) selection limits detection of long and alternatively spliced transcripts compared with rRNA depletion in RNA-SequencingSemantic proximity Read

Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRTSemantic proximity Read

Assessing gene expression profiling from FFPE Patient Samples: A Comparison of Two Library Preparation Approaches and RecommendationsSemantic proximity Read

A ratio-based framework using Quartet reference materials for integrating long- and short-read RNA-seqSemantic proximity Read

A high-efficient capture-based NGS approach for comprehensive analysis of mitochondrial transcriptomSemantic proximity Read