Article

Probe-Free Multiplexed RPA Detection Via Single-Molecule Nanopore Sensing and Deep Learning Classification

2025-04-25

Abstract excerpt

<h4>ABSTRACT</h4> Recombinase Polymerase Amplification (RPA) is a rapid, sensitive, and isothermal method for nucleic acid amplification that has gained widespread use in diagnostic applications. While well-suited for single-target assays, extending RPA to multiplex detection remains technically challenging despite the multiplexed RPA being essential for tasks such as differential diagnosis and inclusion of inter...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: adca160e-d849-5c46-85b8-d085d5ff9506
DOI: 10.1101/2025.04.24.650542

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Probe-Free Multiplexed RPA Detection Via Single-Molecule Nanopore Sensing and Deep Learning ClassificationDOI 10.1101/2025.04.24.650542Read

A nanopore interface for high bandwidth DNA computingSemantic proximity Read

Purely electrical SARS-CoV-2 sensing based on single-molecule countingSemantic proximity Read

Donut PCR: a rapid, portable, multiplexed, and quantitative DNA detection platform with single-nucleotide specificitySemantic proximity Read

Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probesSemantic proximity Read

Rapid, point-of-care molecular diagnostics with Cas13Semantic proximity Read

Highly multiplexed rapid DNA detection with single-nucleotide specificity via convective PCR in a portable device.Semantic proximity Read

Nanoarray Digital Polymerase Chain Reaction with High-Resolution Melt for Enabling Broad Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Test.Semantic proximity Read

Visual “Dam-Break Drainage” Nucleic Acid Chips: Family-Affordable Platform for Rapid and Ultrasensitive Pathogens DetectionSemantic proximity Read

Ultrasensitive electrochemical detection of clinically relevant genetic mutations via nanoparticle surface localized amplification and machine learning.Semantic proximity Read

Machine Learning-Driven Nanopore Sensing for Quantitative, Label-Free miRNA DetectionSemantic proximity Read

Nanopore Identification of Single Nucleotide Mutations in Circulating Tumor DNA by Multiplexed Ligation.Semantic proximity Read

Deep Learning‐Enhanced Hand‐Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPRSemantic proximity Read