Article

Donut PCR: a rapid, portable, multiplexed, and quantitative DNA detection platform with single-nucleotide specificity

2020-04-25

Abstract excerpt

Current platforms for molecular analysis of DNA markers are either limited in multiplexing (qPCR, isothermal amplification), turnaround time (microarrays, NGS), quantitation accuracy (isothermal amplification, microarray, nanopore sequencing), or specificity against single-nucleotide differences (microarrays, nanopore sequencing). Here, we present the Donut PCR platform that features high multiplexing, rapid turna...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 25dd1cdc-0829-571a-b8ac-9141784a5850
DOI: 10.1101/2020.04.24.058453

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Donut PCR: a rapid, portable, multiplexed, and quantitative DNA detection platform with single-nucleotide specificityDOI 10.1101/2020.04.24.058453Read

Highly multiplexed rapid DNA detection with single-nucleotide specificity via convective PCR in a portable device.Semantic proximity Read

Probe-Free Multiplexed RPA Detection Via Single-Molecule Nanopore Sensing and Deep Learning ClassificationSemantic proximity Read

Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.Semantic proximity Read

Evaluation, Validation, and Implementation of the Idylla System as Rapid Molecular Testing for Precision MedicineSemantic proximity Read

Harnessing non-standard nucleic acids for highly sensitive icosaplex (20-plex) detection of microbial threatsSemantic proximity Read

Universal PCR genotyping assay that achieves single copy sensitivity with any primer pair.Semantic proximity Read

Decentralizing genetic testing for biodiversity monitoring and biosurveillance with the Nucleic Acid Barcode Identification Tool (NABIT) and Molecular Development Kit (MDK)Semantic proximity Read

Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental SurveillanceSemantic proximity Read

Development of a single tube 640-plex genotyping method for detection of nucleic acid variations on microarrays.Semantic proximity Read

UbiNAAT: a multiplexed point-of-care nucleic acid diagnostic platform for rapid at-home pathogen detectionSemantic proximity Read

Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing.Semantic proximity Read

Competitive SNP-LAMP probes for rapid and robust single-nucleotide polymorphism detectionSemantic proximity Read