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Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes

14 Nov 2023

Abstract excerpt

We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, with chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity. We show that this sensing strategy can directly detect spike (S) and nucleocapsid (N) proteins in unprocessed human saliva. Moreover, our approach enables the...

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Share to Advanced biosensing and bioanalysis techniques
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PMID: 37963924
DOI: 10.1038/s41467-023-43004-9

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