Article

Low cell number proteomic analysis using in-cell protease digests reveals a robust signature for cell cycle state classification

2020-07-03

Abstract excerpt

Comprehensive proteome analysis of rare cell phenotypes remains a significant challenge. We report a method for low cell number mass spectrometry (MS)-based proteomics using protease digestion of mildly formaldehyde-fixed cells in cellulo , which we call the ‘in-cell digest’. We combined this with AMPL (Averaged MS1 Precursor Library Matching) to quantitatively characterise proteomes from low cell numbers of huma...

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Identifiers and source

Literature Corpus work: ad96e702-5f53-5088-935b-7c4fb568cf62
DOI: 10.1101/2020.07.03.186023

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