Article

Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

2017-04-09

Abstract excerpt

The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al. 2014; Ly et al. 2015). Here we show that by using specific intracellular immunolabeling protocols, FACS separation of interphase and mitotic cells, including mitotic...

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Identifiers and source

Literature Corpus work: e8262287-4762-5f65-8c12-7543e8c6b01f
DOI: 10.1101/125831

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