Article

Dissociation of solid tumour tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses

2019-06-27

Abstract excerpt

<h4>Background</h4> Single-cell RNA sequencing (scRNAseq) is a powerful tool for studying complex biological systems, such as tumour heterogeneity and tissue microenvironments. However, the sources of technical and biological variation in primary solid tumour tissues and patient-derived mouse xenografts for scRNAseq, are not well understood. Here, we used low temperature (6°C) protease and collagenase (37°C) to i...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 76f00dbb-af69-513e-998e-ae1da384d165
DOI: 10.1101/683227

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Dissociation of solid tumour tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responsesDOI 10.1101/683227Read

Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysisSemantic proximity Read

Dissociation Protocols used for Sarcoma Tissues Bias the Transcriptome observed in Single-cell and Single-nucleus RNA sequencingSemantic proximity Read

A strategy to address dissociation-induced compositional and transcriptional bias for single-cell analysis of the human mammary glandSemantic proximity Read

Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysisSemantic proximity Read

Transcriptomic evaluation of immune-infiltrated patient-derived tumor organoids as preclinical models in renal cell carcinomaSemantic proximity Read

Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencingSemantic proximity Read

Lung, spleen and oesophagus tissue remains stable for scRNAseq in cold preservationSemantic proximity Read

<i>ctQC</i> improves biological inferences from single cell and spatial transcriptomics dataSemantic proximity Read

scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservationSemantic proximity Read

A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumorsSemantic proximity Read

Tumour specimen cold ischemia time impacts molecular cancer drug target discoverySemantic proximity Read

FixNCut: Single-cell genomics through reversible tissue fixation and dissociationSemantic proximity Read