Article

Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics

2024-03-20

Abstract excerpt

<title>Abstract</title> <p>Top-down proteomics (TDP) has seen significant advances in the past, and a plethora of sample preparation workflows have been developed. Here, we systematically investigated the influence of different sample preparation steps on proteoform and protein identifications, including cell lysis, reduction and alkylation, proteoform enrichment, purification, and fractionation. We found that al...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: abcd1e4f-583a-514f-a895-19773abe5546
DOI: 10.21203/rs.3.rs-3990966/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down ProteomicsDOI 10.21203/rs.3.rs-3990966/v1Read

Integrated top-down and bottom-up mass spectrometry enables precise characterization of proteoforms and their post-translational modifications within the protein coronaSemantic proximity Read

Proteoforms as the next proteomics currency.Semantic proximity Read

Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid ExtractionSemantic proximity Read

The Human Proteoform Atlas: a FAIR community resource for experimentally derived proteoforms.Semantic proximity Read

Proteoform Patterns in the Samples of Hepatocellular Carcinoma: Aspects of OncomarkersSemantic proximity Read

Mapping intact protein isoforms in discovery mode using top-down proteomics.Semantic proximity Read

Proteomics Techniques in Focus: From Basics to Advanced ApplicationsSemantic proximity Read

Integral-Omics: serial extraction and profiling of metabolome, lipidome, genome, transcriptome, whole proteome and phosphoproteome using biopsy tissueSemantic proximity Read

Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkersSemantic proximity Read

Qualitative and quantitative top-down proteomics of human colorectal cancer cell lines identified 23000 proteoforms and revealed drastic proteoform-level differences between metastatic and non-metastatic cancer cellsSemantic proximity Read

Integral-Omics: Serial Extraction and Profiling of Metabolome, Lipidome, Genome, Transcriptome, Whole Proteome and Phosphoproteome Using Biopsy TissueSemantic proximity Read

Mapping the Human Ghost Proteome: Classification and Experimental Detection Biases in the Identification of Alternative MicroproteinsSemantic proximity Read