Article

Deletion of CnLIG4 DNA ligase gene in the fungal pathogen Cryptococcus neoformans elevates homologous recombination efficiency

2010-01-01

Abstract excerpt

In eukaryotes from yeasts to human, DNA double-strand breaks are repaired by nonhomologous end-joining (NHEJ) or homologous integration (HI). In the human pathogenic yeast Cryptococcus neoformans, gene manipulation by HI does not occur frequently because ectopic integration by NHEJ is predominant, and it has been necessary to screen 30-100 transformants per experiment to obtain transformants with the desired genot...

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Literature Corpus work: aa86a281-9337-589c-a853-99ed757d2211
DOI: 10.1007/s10267-009-0001-7

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