ASSET: A Framework for Decoding Aptamer Specificity of an Enriched Library by Next-Generation Sequencing of Experimental Samples

Abstract excerpt

<h4>ABSTRACT</h4> The SELEX process to identify RNA and DNA aptamers relies on sequencing selection rounds to detect highly specific aptamers through patterns of aptamer accumulation or enrichment. However, this approach infers rather than quantify aptamer specificity. Here we present a novel strategy for directly quantifying aptamer specificity within enriched libraries termed Aptamer Specificity Sequencing for...

Topics

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• Aptamers, Nucleotide
• High-Throughput Nucleotide Sequencing
• Ligands
• Nucleic Acid Conformation
• Plasminogen Activator Inhibitor 1
• RNA
• Sequence Alignment
• Sequence Analysis, RNA

Identifiers and source

Literature Corpus work

a3d3502c-edd6-5ef4-abc5-5f56aec98047

DOI

10.1101/2025.08.27.672406