Article

ABACUS: A flexible UMI counter that leverages intronic reads for single-nucleus RNAseq analysis

2020-11-16

Abstract excerpt

Single-nucleus RNA sequencing (sNuc-RNAseq) is an emerging powerful genomics technology that combines droplet microfluidics with next-generation sequencing to interrogate transcriptome changes at single nucleus resolution. Here we developed Abacus, a flexible UMI counter software for sNuc-RNAseq analysis. Abacus draws extra information from sequencing reads mapped to introns of pre-mRNAs (~60% of total data) that...

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Literature Corpus work: 9a5807eb-b0c9-5d3d-a56c-d762c4a7affa
DOI: 10.1101/2020.11.13.381624

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