Article

Optimizing culture-free approaches to recover high quality <i>M. tuberculosis</i> genomic variation

2025-12-18

Abstract excerpt

<h4>Background:</h4> Mycobacterium tuberculosis ( Mtb ) genomic epidemiology often relies on culturing patient sputum, a time and labor-intensive process. Hybrid capture approaches have been successfully used to enrich Mtb DNA from complex clinical samples, yet the accuracy of variant identification from captured samples has not been systematically evaluated. <h4>Methods:</h4> We created artificial strain mix...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 94685028-c6dc-5128-8345-65e12e9f3b36
DOI: 10.64898/2025.12.16.25342406

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimizing culture-free approaches to recover high quality <i>M. tuberculosis</i> genomic variationDOI 10.64898/2025.12.16.25342406Read

Optimizing culture-free approaches to recover high-quality Mycobacterium tuberculosis genomic variation.Semantic proximity Read

Direct nanopore sequencing of <i>M. tuberculosis</i> on sputa and rescue of suboptimal results to enhance transmission surveillanceSemantic proximity Read

A new method for detecting mixed <i>Mycobacterium tuberculosis</i> infection and reconstructing constituent strains provides insights into transmissionSemantic proximity Read

Whole genome sequencing <i>Mycobacterium tuberculosis</i> directly from sputum identifies more genetic diversity than sequencing from cultureSemantic proximity Read

Comprehensive and accurate genetic variant identification from contaminated and low coverage <i>Mycobacterium tuberculosis</i> whole genome sequencing dataSemantic proximity Read

Genetic diversity within diagnostic sputum samples is mirrored in the culture of <i>Mycobacterium tuberculosis</i>Semantic proximity Read

In-host population dynamics of <i>M. tuberculosis</i> during treatment failureSemantic proximity Read

A new method for detecting mixed Mycobacterium tuberculosis infection and reconstructing constituent strains provides insights into transmissionSemantic proximity Read

Evaluation of <i>Mycobacterium Tuberculosis</i> enrichment in metagenomic samples using ONT adaptive sequencing and amplicon sequencing for identification and variant callingSemantic proximity Read

TBtypeR: Sensitive detection and sublineage classification of low-frequency<i>Mycobacterium tuberculosis</i>complex mixed infectionsSemantic proximity Read

A needle in a haystack: metagenomic DNA sequencing to quantify <i>Mycobacterium tuberculosis</i> DNA and diagnose tuberculosisSemantic proximity Read

Effects of Host, Sample, and in vitro Culture on Genomic Diversity of Pathogenic MycobacteriaSemantic proximity Read