Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimizing culture-free approaches to recover high-quality Mycobacterium tuberculosis genomic variation.

Microbial genomics - 1 Aug 2026

Walter Katharine S, Dos Santos Paulo César Pereira, Carey Allison, Shakir Salika M, Colijn Caroline, Cohen Ted, Mathema Barun, Croda Julio, Andrews Jason R

Abstract excerpt

Background. Mycobacterium tuberculosis (Mtb) genomic epidemiology often relies on culturing patient sputum, a time- and labour-intensive process. Hybrid capture approaches have been successfully used to enrich Mtb DNA from complex clinical samples, yet the accuracy of variant identification from captured samples has not been systematically evaluated.Methods. We created artificial strain mixtures of two...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA, Bacterial
Share to Gene Frequency

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Variation

Share to Genome, Bacterial

Share to Mycobacterium tuberculosis

Share to Sputum

Share to Tuberculosis

Share to Whole Genome Sequencing

Source

Open in PubMed Open full text

Identifiers

PMID: 42611044
DOI: 10.1099/mgen.0.001806
PMCID: PMC13485273

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimizing culture-free approaches to recover high-quality Mycobacterium tuberculosis genomic variation.PMID 42611044Read ↗

Optimizing culture-free approaches to recover high quality <i>M. tuberculosis</i> genomic variationSemantic proximity Read ↗

A new method for detecting mixed Mycobacterium tuberculosis infection and reconstructing constituent strains provides insights into transmissionSemantic proximity Read ↗

A new method for detecting mixed <i>Mycobacterium tuberculosis</i> infection and reconstructing constituent strains provides insights into transmissionSemantic proximity Read ↗

Assessment and validation of enrichment and target capture approaches to improve Mycobacterium tuberculosis WGS from direct patient samplesSemantic proximity Read ↗

Whole-Genome Sequencing Exhibits Better Diagnostic Performance than Variable-Number Tandem Repeats for Identifying Mixed Infections of Mycobacterium tuberculosis.Semantic proximity Read ↗

Identifying mixed Mycobacterium tuberculosis infections from whole genome sequence data.Semantic proximity Read ↗

Effects of Host, Sample, and in vitro Culture on Genomic Diversity of Pathogenic MycobacteriaSemantic proximity Read ↗

Whole genome sequencing Mycobacterium tuberculosis directly from sputum identifies more genetic diversity than sequencing from cultureSemantic proximity Read ↗

Detection of Low-Level Mixed-Population Drug Resistance in Mycobacterium tuberculosis Using High Fidelity Amplicon Sequencing.Semantic proximity Read ↗

Comprehensive and accurate genetic variant identification from contaminated and low-coverage Mycobacterium tuberculosis whole genome sequencing dataSemantic proximity Read ↗

Genetic diversity within diagnostic sputum samples is mirrored in the culture of Mycobacterium tuberculosis across different settings.Semantic proximity Read ↗

Comprehensive and accurate genetic variant identification from contaminated and low coverage <i>Mycobacterium tuberculosis</i> whole genome sequencing dataSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.