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Whole genome sequencing <i>Mycobacterium tuberculosis</i> directly from sputum identifies more genetic diversity than sequencing from culture

2018-10-18

Abstract excerpt

<h4>Background</h4> Repeated culture reduces within-sample Mycobacterium tuberculosis genetic diversity due to selection of clones suited to growth in culture and/or random loss of lineages, but it is not known to what extent omitting the culture step altogether alters genetic diversity. We compared M. tuberculosis whole genome sequences generated from 33 paired clinical samples using two methods. In one method...

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Literature Corpus work
1104a175-d26c-53bb-8b86-8f39a79ead8d
DOI
10.1101/446849
Open publication

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Whole genome sequencing <i>Mycobacterium tuberculosis</i> directly from sputum identifies more genetic diversity than sequencing from cultureDOI 10.1101/446849
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