Article
Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes.
Scientific reports - 24 Aug 2017
Ishizu Takamaru, Higo Shuichiro, Masumura Yuki, Kohama Yasuaki, Shiba Mikio, Higo Tomoaki, Shibamoto Masato, Nakagawa Akito, Morimoto Sachio, Takashima Seiji, Hikoso Shungo, Sakata Yasushi
Abstract excerpt
Although high-throughput sequencing can elucidate the genetic basis of hereditary cardiomyopathy, direct interventions targeting pathological mutations have not been established. Furthermore, it remains uncertain whether homology-directed repair (HDR) is effective in non-dividing cardiomyocytes. Here, we demonstrate that HDR-mediated genome editing using CRISPR/Cas9 is effective in non-dividing cardiomyocytes....
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