Article

Novel lentiviral constructs for the specification of rare and ectopic cell types in human colonoids

2025-10-16

Abstract excerpt

Human colonoids derived from adult intestinal tissue provide powerful models for epithelial biology but lack several rare or regionally restricted cell types. To overcome this limitation, we engineered doxycycline-inducible lentiviral constructs to drive expression of transcription factors that control lineage specification, including KLF4, POU2F3, SPIB, NEUROG3, and NEUROG3 T2A NKX6-3. Transduction of human colon...

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Literature Corpus work: 90623106-73cd-56e8-abb6-a653f5e7405b
DOI: 10.1101/2025.10.16.680258

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