Article

Culture wars: Empirically determining the best approach for plasmid library amplification

2024-05-27

Abstract excerpt

DNA libraries are critical components of many biological assays. These libraries are often kept in plasmids that are amplified in E. coli to generate sufficient material for an experiment. Library uniformity is critical for ensuring that every element in the library is tested similarly, and is thought to be influenced by the culture approach used during library amplification. We tested five commonly used culturin...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8e065a0b-ea64-584a-8c95-0249c8a42249
DOI: 10.1101/2024.05.24.595835

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Culture wars: Empirically determining the best approach for plasmid library amplificationDOI 10.1101/2024.05.24.595835Read

Automation workflow for high-throughput arrayed plasmid DNA preparation and quantificationSemantic proximity Read

Template selection during manipulation of complex mixtures by PCR.Semantic proximity Read

Recovery of small plasmid sequences via Oxford Nanopore sequencingSemantic proximity Read

Nanopore adaptive sampling effectively enriches bacterial plasmidsSemantic proximity Read

Sequencing Strategy to Ensure Accurate Plasmid AssemblySemantic proximity Read

Rapid isolation of rare clones from highly complex DNA libraries by PCR analysis of liquid gel poolsSemantic proximity Read

Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.Semantic proximity Read

Determination of a Screening Metric for High Diversity DNA Libraries.Semantic proximity Read

High molecular weight DNA extraction strategies for long-read sequencing of complex metagenomesSemantic proximity Read

Strong spurious transcription likely a cause of DNA insert bias in typical metagenomic clone librariesSemantic proximity Read

Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomesSemantic proximity Read

PlasmidHunter: Accurate and fast prediction of plasmid sequences using gene content profile and machine learningSemantic proximity Read