Article
Template selection during manipulation of complex mixtures by PCR.
BioTechniques - 1 Apr 2001
Teng D H, Hsu F, Peterson I, Cardon K E, Caponigro G, Kamb A
Abstract excerpt
PCR is ubiquitous in molecular biology. It is used to amplify single sequences from large genomes, or populations of sequences from complex mixtures such as cDNA libraries in mammalian cells. These cDNA libraries are often employed in subsequent labor-intensive experiments such as genetic screens, the outcome of which depends on library quality. The use of PCR to amplify diverse sequence populations raises...
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