Article

Dissecting mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage

2020-08-06

Abstract excerpt

Mutational signatures are imprints of pathophysiological processes arising through tumorigenesis. Here, we generate isogenic CRISPR-Cas9 knockouts (Δ) of 43 genes in human induced pluripotent stem cells, culture them in the absence of added DNA damage, and perform wholegenome sequencing of 173 daughter subclones. Δ OGG1 , Δ UNG , Δ EXO1 , Δ RNF168 , Δ MLH1 , Δ MSH2 , Δ MSH6 , Δ PMS1 , and Δ PMS2 produce m...

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Identifiers and source

Literature Corpus work: 8dafd887-1f98-5ed0-ba64-b6c18da35c03
DOI: 10.1101/2020.08.04.234245

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