Article

A SMART method for efficiently isolating monoclonal antibodies from individual rhesus macaque memory B cells

2023-06-05

Abstract excerpt

Characterizing antigen-specific B cells is a critical component of vaccine and infectious disease studies in rhesus macaques (RMs). However, it is challenging to capture immunoglobulin variable (IgV) genes from individual RM B cells using 5’ multiplex (MTPX) primers in nested PCR reactions. In particular, the diversity within RM IgV gene leader sequences necessitates the use of large 5’ MTPX primer sets to amplify...

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Literature Corpus work: 8daa1ac7-bf6a-5e3f-9fb1-90cdb72243c8
DOI: 10.1101/2023.06.02.543510

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