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Efficient Methods To Isolate Human Monoclonal Antibodies from Memory B Cells and Plasma Cells

19 Sept 2014

Abstract excerpt

In this article, we highlight the advantages of isolating human monoclonal antibodies from the human memory B cells and plasma cell repertoires by using high-throughput cellular screens. Memory B cells are immortalized with high efficiency using Epstein-Barr virus (EBV) in the presence of a toll-like receptor (TLR) agonist, while plasma cells are maintained in single-cell cultures by using interleukin 6 (IL-6) or...

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PMID: 26104354
DOI: 10.1128/microbiolspec.aid-0018-2014

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