Article

Interface-guided phenotyping of coding variants in the transcription factor RUNX1 with SEUSS

2023-08-04

Abstract excerpt

Understanding the consequences of single amino acid substitutions in cancer driver genes remains an unmet need. Perturb-seq provides a tool to investigate the effects of individual mutations on cellular programs. Here we deploy SEUSS, a Perturb-seq like approach, to generate and assay mutations at physical interfaces of the RUNX1 Runt domain. We measured the impact of 115 mutations on RNA profiles in single myelog...

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Literature Corpus work: 8a37115e-4c7c-5b0a-8e04-5e172b5bf2bb
DOI: 10.1101/2023.08.03.551876

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