Article

High-efficiency HDR in immortalized cell lines by crude rAAV mediated donor template delivery

2022-05-03

Abstract excerpt

Owing to low efficiency of homology-directed repair (HDR), precise knock-in (KI) of large DNA fragments is a challenge in genome editing. High-efficiency HDR has been reported for primary cells in preclinical gene therapy by combining CRISPR/Cas9 mediated induction of double-strand breaks (DSB) with delivery of a single-stranded DNA HDR-donor-template via highly purified recombinant adeno-associated virus (rAAV)....

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Literature Corpus work: 8237484b-1c69-57f2-a9b2-38a384f70253
DOI: 10.1101/2022.05.02.490359

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