Article

EASTR: Correcting systematic alignment errors in multi-exon genes

2023-05-12

Abstract excerpt

Accurate alignment of transcribed RNA to reference genomes is a critical step in the analysis of gene expression, which in turn has broad applications in biomedical research and in the basic sciences. We have discovered that widely used splice-aware aligners, such as STAR and HISAT2, can introduce erroneous spliced alignments between repeated sequences, leading to the inclusion of falsely spliced transcripts in RN...

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Literature Corpus work: 81738177-5b77-5638-8728-c3de1465921d
DOI: 10.1101/2023.05.10.540179

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