Article

Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanning

2024-08-06

Abstract excerpt

Deep mutational scanning (DMS) has emerged as a powerful approach for evaluating the effects of mutations on binding or function. Here, we developed a multiplexed DMS by phage display protocol to define the binding determinants of short linear motifs (SLiMs) binding to peptide binding domains. We first designed a benchmarking DMS library to evaluate the performance of the approach on well-known ligands for eleven...

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Literature Corpus work: 8147b415-fa89-55e8-9917-bc82ba84909b
DOI: 10.1101/2024.08.06.606761

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