Article

A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells

2025-07-16

Abstract excerpt

Current methods to engineer antigen-specific receptors rely on randomly integrating vectors or double-strand break induced targeted integration, both of which pose safety risks. To implement an all-in-one tool for multiplex knockout (KO) and knock in (KI), we expand the use of cytosine and adenine base editor (ABE) nickase activity to stimulate homology-directed repair (HDR) and insert clinically relevant chimeric...

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Literature Corpus work: 7ff83d8f-a121-5287-8cf5-ce14d20a69d8
DOI: 10.1101/2025.07.11.664404

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