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Recombinant Protein Spectral Library (rPSL) DIA-MS Method Improves Identification and Quantification of Low-Abundance Cancer-Associated and Kynurenine Pathway Proteins

2024-11-29

Abstract excerpt

<title>Abstract</title> <p>Data-independent acquisition mass spectrometry (DIA-MS) is a powerful tool for quantitative proteomics, but a well-constructed reference spectral library is crucial to optimize DIA analysis, particularly for low-abundance proteins. In this study, we evaluate the efficacy of a recombinant protein spectral library (rPSL), generated from tryptic digestion of 42 human recombinant proteins,...

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Literature Corpus work: 7eb1f74f-3c15-5dd9-a3cc-8c32df2c16fd
DOI: 10.21203/rs.3.rs-5364803/v1

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