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Prediction of Transcription Factor DNA Binding Affinity with High-Throughput <i>K</i> <sub>d</sub> Measurements and Deep Learning

2026-05-20

Abstract excerpt

Transcription factors (TFs) regulate gene expression through specific interactions with genomic DNA. While TF binding motifs from public databases describe sequence preferences, quantifying genome-wide affinity ( K d ) is highly desirable for a more accurate thermodynamic description. Here, we report ivt FOODIE ( in vitro FOOtprinting with DeamInasE), an assay that leverages deaminase-mediated cytosine-to-uraci...

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Literature Corpus work: 72c52760-2993-5b12-bd03-35aa398681b0
DOI: 10.64898/2026.05.18.725930

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