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Article

5’XP sRNA-seq: Efficient Identification of Transcripts With and Without 5’ Phosphorylation Reveals Evolutionary Conserved Small RNA

2020-08-23

Abstract excerpt

<h4>ABSTRACT</h4> Small RNA (sRNA) sequencing has been critical for our understanding of many cellular processes, including gene regulation. Nonetheless, the varying biochemical properties of sRNA, such as 5’ nucleotide modifications, makes many sRNA subspecies incompatible with common protocols for sRNA sequencing. Here we describe 5XP-seq that outlines a novel strategy that solves this problem. By tagging 5’P s...

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Literature Corpus work
71f7604a-bd69-5813-a8bb-7639c07888f9
DOI
10.1101/2020.08.21.261412
Open publication

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5’XP sRNA-seq: Efficient Identification of Transcripts With and Without 5’ Phosphorylation Reveals Evolutionary Conserved Small RNADOI 10.1101/2020.08.21.261412
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