Article
5’XP sRNA-seq: Efficient Identification of Transcripts With and Without 5’ Phosphorylation Reveals Evolutionary Conserved Small RNA
2020-08-23
Abstract excerpt
<h4>ABSTRACT</h4> Small RNA (sRNA) sequencing has been critical for our understanding of many cellular processes, including gene regulation. Nonetheless, the varying biochemical properties of sRNA, such as 5’ nucleotide modifications, makes many sRNA subspecies incompatible with common protocols for sRNA sequencing. Here we describe 5XP-seq that outlines a novel strategy that solves this problem. By tagging 5’P s...
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Identifiers and source
- Literature Corpus work
- 71f7604a-bd69-5813-a8bb-7639c07888f9
- DOI
- 10.1101/2020.08.21.261412
