Article

Structural requirements for conformational changes and substrate binding of the human facilitative glucose transporter-GLUT3

1997-01-01

Abstract excerpt

The role of proline residues in transport catalysis mediated by GLUT3 has been investigated using site-directed mutagenesis. PCR methodology was used to construct a series of eight mutant GLUTS cDNAs in which individual proline residues were replaced with alanine. Heterologous expression of the GLUTS Pro-Ala mutants in Xenopus laevis oocytes was achieved by microinjection of in vitro transcribed mRNA and has enabl...

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Literature Corpus work: 6d5dee6c-7d9c-51cf-aaec-a4ec64f0771d
DOI: 10.5525/gla.thesis.71640

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