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Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylation

2025-06-09

Abstract excerpt

Glycosylation of proteins can impact their folding, stability, trafficking and enzymatic activity. Human Gb3/CD77 synthase (α1,4-galactosyltransferase, A4galt) has two occupied N-glycosylation sites. Previously, we demonstrated that the activity of recombinant enzyme relies on its N-glycosylation. In this study, we produced soluble recombinant catalytic domain of human Gb3/CD77 synthase in two expression hosts kno...

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Literature Corpus work
5c8877f4-2fb8-5ed5-9eef-25aec041b31e
DOI
10.1101/2025.06.09.658241
Open publication

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Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylationDOI 10.1101/2025.06.09.658241
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