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Chinese hamster ovary (<scp>CHO</scp>) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialyltransferase expression

13 Jan 2015

Abstract excerpt

N-Glycans of human proteins possess both α2,6- and α2,3-linked terminal sialic acid (SA). Recombinant glycoproteins produced in Chinese hamster overy (CHO) only have α2,3-linkage due to the absence of α2,6-sialyltransferase (St6gal1) expression. The Chinese hamster ST6GAL1 was successfully overexpressed using a plasmid expression vector in three recombinant immunoglobulin G (IgG)-producing CHO cell lines. The...

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Identifiers

PMID: 25641927
DOI: 10.1002/btpr.2038

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