Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function

Abstract excerpt

Natural killer (NK) cells are emerging as a promising platform for engineered adoptive cell therapies. However, gene editing in NK cells remains challenging, and more effective strategies are needed. Here, we established a robust, feeder-free, and modular workflow for genome engineering in primary human NK cells, combining CRISPR/Cas9 with AAV6-mediated transgene delivery. Efficient site-specific transgene integra...

Topics

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• CAR-T cell therapy research
• CRISPR and Genetic Engineering
• Immune Cell Function and Interaction
• RNA Interference and Gene Delivery
• T-cell and B-cell Immunology
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

593cd6ae-68c7-51ff-9de0-fad3c98dbd94

DOI

10.1101/2025.10.05.680386