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A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy

2018-09-29

Abstract excerpt

Tumors can evade natural killer (NK) cells by activating inhibitory pathways. We therefore have developed a highly efficient CRISPR/Cas9-based method for editing the genome of peripheral blood human NK cells (PB-NKs) to knock out ADAM17 and PD1 or knock-in genes using recombinant AAV6. Our method allows editing of PB-NKs at efficiencies reaching 90%, equivalent to methods reported for primary human T cells. Moreov...

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Literature Corpus work: 248b733c-56db-598d-9a56-e2e091b636dc
DOI: 10.1101/430553

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