Article

Truncated Human IRF-1 (1-233) protein shows neutral transcriptional effect on expression of Bax, survivin, Bcl2 and cyclin D1 genes in HEK293T cell line

2022-06-13

Abstract excerpt

<title>Abstract</title> <p>We generated the truncated IRF-1(1-233 amino acids), lacks total C-terminal domains, like the Transactivation domain, instability domain, Multifunctional domains. We used semi-nested PCR technique followed by gene cloning in <italic>E.coli</italic> and observed profound expression of mutant GST-IRF-1 protein (~ 52 kDa) in 12.5% SDS PAGE. We ectopically expressed truncated mutant version...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5862d626-a112-5d77-8a51-194e8d153992
DOI: 10.21203/rs.3.rs-1749803/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Truncated Human IRF-1 (1-233) protein shows neutral transcriptional effect on expression of Bax, survivin, Bcl2 and cyclin D1 genes in HEK293T cell lineDOI 10.21203/rs.3.rs-1749803/v1Read

Function and mechanism by which interferon regulatory factor-1 inhibits oncogenesisSemantic proximity Read

Cutting Edge: IRF8 Regulates Bax Transcription In Vivo in Primary Myeloid CellsSemantic proximity Read

Association Study of BIF-1 Gene Expression with Histopathological Characteristics and Hormone Receptors in Breast CancerSemantic proximity Read

Loss of transcription factor IRF-1 affects tumor susceptibility in mice carrying the Ha-ras transgene or nullizygosity for p53.Semantic proximity Read

A conserved intronic response element mediates direct p53-dependent transcriptional activation of both the human and murine bax genesSemantic proximity Read

BHRF1, a viral homologue of the Bcl-2 oncogene, is conserved at both the sequence and functional level in different Epstein-Barr virus isolates.Semantic proximity Read

Bim Nuclear Translocation and Inactivation by Viral Interferon Regulatory FactorSemantic proximity Read

Ectopic Expression of Interferon Regulatory Factor-1 Promotes Human Breast Cancer Cell Death and Results in Reduced Expression of SurvivinSemantic proximity Read

Transcriptional regulation by a point mutant of adenovirus-2 E1a product lacking DNA binding activity.Semantic proximity Read

Interferon regulatory factor 1 (IRF-1) mediates cell growth inhibition by transactivation of downstream target genesSemantic proximity Read

p53-dependent repression of the human MCL-1 gene encoding an anti-apoptotic member of the BCL-2 family: the role of Sp1 and of basic transcription factor binding sites in the MCL-1 promoter.Semantic proximity Read