Article

Npl4 decodes polyubiquitin length and gates D1-D2 coupling in human VCP/p97

2026-05-13

Abstract excerpt

<title>Abstract</title> <p> VCP/p97 binds the Npl4 <bold>–</bold> Ufd1 heterodimer adaptor to extract polyubiquitinated substrates for proteasomal degradation, but how it decodes K48-linked chain length and how D1-coupled events license downstream D2 power strokes remain unclear. Here we introduce smUbiRAD, or single-molecule ubiquitin recognition and dynamics, and identify a sharp chain-length threshold: Npl4...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5716cf31-a06c-510e-8863-85e4fe802c26
DOI: 10.21203/rs.3.rs-9588629/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Npl4 decodes polyubiquitin length and gates D1-D2 coupling in human VCP/p97DOI 10.21203/rs.3.rs-9588629/v1Read

Npl4 decodes polyubiquitin length and gates D1-D2 coupling in human VCP/p97Semantic proximity Read

Comprehensive approach to study branched ubiquitin chains reveals roles for K48-K63 branches in VCP/p97-related processesSemantic proximity Read

ASPL-driven subunit exchange remodels VCP/p97 hexamers and is impaired by a multisystem proteinopathy mutationSemantic proximity Read

Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP•NPLOC4•UFD1L1 is enhanced by a mutation that causes multisystem proteinopathySemantic proximity Read

The VCP/p97 system at a glance: connecting cellular function to disease pathogenesisSemantic proximity Read

Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP•NPLOC4•UFD1L is enhanced by a mutation that causes multisystem proteinopathy.Semantic proximity Read

Faf1 accelerates p97-mediated protein unfolding by promoting ubiquitin engagementSemantic proximity Read

Valosin-Containing Protein (VCP)/p97 Oligomerization.Semantic proximity Read

The N-terminal Region of the Ubiquitin Regulatory X (UBX) Domain-containing Protein 1 (UBXD1) Modulates Interdomain Communication within the Valosin-containing Protein p97.Semantic proximity Read

Interaction between the AAA+ ATPase p97 and its cofactor ataxin3 in health and disease: Nucleotide-induced conformational changes regulate cofactor binding.Semantic proximity Read

Emerging functions of the VCP/p97 AAA-ATPase in the ubiquitin system.Semantic proximity Read

Conformation-specific Antibody Deciphers K27-linked Ubiquitination in Chaperone-Mediated ProteostasisSemantic proximity Read