Article

Npl4 decodes polyubiquitin length and gates D1-D2 coupling in human VCP/p97

2026-05-05

Abstract excerpt

VCP/p97 binds the Npl4 – Ufd1 heterodimer adaptor to extract polyubiquitinated substrates for proteasomal degradation, but how it decodes K48-linked chain length and how D1-coupled events license downstream D2 power strokes remain unclear. Here we introduce smUbiRAD, or single-molecule ubiquitin recognition and dynamics, and identify a sharp chain-length threshold: Npl4 binds transiently to short chains but switc...

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Literature Corpus work: 193808b2-0364-51a3-ab1a-0b3a5b7a72b3
DOI: 10.64898/2026.05.01.722286

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