Article

Anti-cancer drug-induced mitochondrial alteration becomes a limitation of metabolic viability-based MTT assay in analyzing growth inhibition

2023-09-21

Abstract excerpt

<title>Abstract</title> <p>Background High throughput metabolic viability-based colorimetric MTT assay is widely used for cytotoxicity screening of various chemical compounds, anti-neoplastic drugs, and other chemotherapeutic agents. The yellow MTT tetrazolium salt reduces to purple formazan crystals, predominantly by mitochondrial dehydrogenases. The assay assumes all cells have a similar number of mitochondria...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5538ae13-b2f3-542d-9bf2-9379f94e947d
DOI: 10.21203/rs.3.rs-3354673/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Anti-cancer drug-induced mitochondrial alteration becomes a limitation of metabolic viability-based MTT assay in analyzing growth inhibitionDOI 10.21203/rs.3.rs-3354673/v1Read

Functional linkage between mitochondrial electron transport, glycolysis, and AMP-activated protein kinase signaling underlying cancer cell survivalSemantic proximity Read

Comprehensive review on how platinum- and taxane-based chemotherapy of ovarian cancer affects biology of normal cellsSemantic proximity Read

Cellular Discrepancy of Platinum Complexes in Interfering with Mitochondrial DNASemantic proximity Read

Aberrant ROS Served as an Acquired Vulnerability of Cisplatin-resistant Lung CancerSemantic proximity Read

The role of mitochondria in cellular toxicity as a potential drug targetSemantic proximity Read

Resveratrol inhibits HeLa cell proliferation by regulating mitochondrial functionSemantic proximity Read

Comparative Cytotoxic and Apoptotic Effects of drugs Compounds on Human Cell LinesSemantic proximity Read

Mitochondria: A Promising Target for Anticancer AlkaloidsSemantic proximity Read

In vitro assays and techniques utilized in anticancer drug discoverySemantic proximity Read

In Vitro Cell Death Discrimination and Screening Method by Simple and Cost-Effective Viability AnalysisSemantic proximity Read

Metabolic vulnerability of cisplatin‐resistant cancersSemantic proximity Read

Phenotypic screen for oxygen consumption rate identifies an anti-cancer naphthoquinone that induces mitochondrial oxidative stress.Semantic proximity Read