Article

Vector copy number analysis using droplet digital PCR method in hematopoietic stem cells and T lymphocytes after lentiviral transduction: optimization and limitations

2024-01-31

Abstract excerpt

In the context of ex vivo gene therapy or CAR-T cell therapy, vector copy number (VCN) analysis in transduced cells by lentiviral vectors enables the assessment of risk and therapeutic efficiency in patients. In this study, we show that a ddPCR-based method can replace easily a TaqMan qPCR assay for VCN analysis, by measuring the number of pro-viral DNA copies per host cell genome in blood samples. Firstly, we hav...

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Literature Corpus work: 4f6b0c6b-4f64-539e-9b68-e8cb14c89885
DOI: 10.22541/au.170667475.57467454/v1

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